Chromosome Banding in Crustacea. I. Karyotype, Ag-NORs, C Banding and Treatment with EcoRI, PstI and KpnI Restriction Endonucleases in Artemia franciscana

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Karyotype Analysis and Chromosome Banding

Nearly all methods of chromosome banding rely on harvesting chromosomes in mitosis. This is usually achieved by treating cells with tubulin inhibitors, such as colchicine or demecolcine (colcemid), that depolymerize the mitotic spindle and so arrest the cell at this stage. Excessively long incubations with Colcemid result in overcondensed chromosomes that band poorly and moreover some cell type...

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Chromosome markers in Mus musculus: strain differences in C-banding.

The mitotic chromosomes of several inbred strains of mice and a series of F(1) hybrids have been analyzed by quinacrine staining and further characterized by the centromeric heterochromatin banding (C-banding). Inbred strains had the same amount of C-banding material on homologous chromosomes but showed variation in the amount on different chromosomes. F(1) hybrids showed characteristics of eac...

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C-banding karyotype and relationship of the dipodids Allactaga and Jaculus (Mammalia: Rodentia) in Egypt.

The C-banding karyotype of the jerboas Allactaga tetradactyla, Jaculus jaculus jaculus, and Jaculus orientalis was described and interspecific relationships were discussed. Despite the conservation of a relatively small amount of C-heterochromatin located at the centromeric region of some chromosomes in all karyotypes, a striking loss of C-heterochromatin was clearly observed in J. orientalis. ...

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The reactions of the EcoRi and other restriction endonucleases.

The reaction of the EcoRI restriction endonuclease was studied with both the plasmid pMB9 and DNA from bacteriophage lambda as the substrates. With both circular and linear DNA molecules, the only reaction catalysed by the EcoRI restriction endonuclease was the hydrolysis of the phosphodiester bond within one strand of the recognition site on the DNA duplex. The cleavage of both strands of the ...

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Artemia franciscana

Artemia franciscana C. Drewes (updated, 2002) http://www.zool.iastate.edu/~c_drewes/ http://www.zool.iastate.edu/~c_drewes/Artemph.jpg Taxonomy Phylum: Arthropoda Subphylum): Crustacea Class: Branchiopoda (includes fairy shrimp, brine shrimp, daphnia, clam shrimp, tadpole shrimp) Order: Anostraca (brine shrimp and fairy shrimp) Genus and species: Artemia franciscana (= the North American versio...

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ژورنال

عنوان ژورنال: Biota Amazônia

سال: 2014

ISSN: 2179-5746

DOI: 10.18561/2179-5746/biotaamazonia.v4n2p15-19